Hemoglobin Tokyo II [α89 (FG1) His→Asn] : A New Hemoglobin Variant Characterized by Endoproteinase Asp-N Digestion and Collision-Induced Dissociation
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概要
- 論文の詳細を見る
The presence of a hemoglobin mutant was suggested in a male subject who showed an apparently false level of glycated hemoglobin based on ion-exchange chromatography. Electrospray ionization mass spectrometry detected a mutant molecule with a mass of 15, 103 units, 23 units less than normal α-globin. The mutation was identified in a large tryptic peptide, αT9, of residues 62-90. Endoproteinase Asp-N digestion of carboxymethylated α-globin cleaved the region into peptides of ca. 1,000 Da, and localized the mutation to the peptide corresponding to residues 85-94. Collision-induced dissociation of the mutated peptide identified an amino acid substitution, Hisrarr;Asn, at position 89. This is a newly described mutation and the mutant was named Hb Tokyo II.
- 日本質量分析学会の論文
- 2000-10-01
著者
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Wada Yoshinao
Department Of Molecular Medicine Osaka Medical Center
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NAKANO Tomokazu
Department of Molecular Medicine, Osaka Medical Center and Research Institute for Maternal and Child
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Nakano Tomokazu
Department Of Molecular Medicine Osaka Medical Center And Research Institute For Maternal And Child
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