Determination of the rdw Rat Genotype by Polymerase Chain Reaction with Allele-Specific Primer (PCR-ASP)

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概要

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• The rat carrying the rdw mutation (rdw rat) is a dwarf mutant with hypothyroidism that is caused by a single G to C transversion in the thyroglobulin gene. Therefore, the development of a simple method for molecular-based genotyping of this mutation has been problematic. We have developed a rapid and simple genotyping method that provides identification of both the rdw and wild-type allele. This polymerase chain reaction with an allele-specific primer (PCR-ASP) method amplifies only the specific allele, wild or mutant type, by using 3-terminal mismatched primers that pair only with the respective alleles. This assay should be of value for rdw colony control and rapid discrimination of rdw/rdw, rdw/+ and +/+ rats.

• 社団法人　日本実験動物学会の論文

著者

• Takamatsu Ken

  Department Of Biophysics And Biochemistry Faculty Of Science Universiiy Of Tokyo

• Umezu Motoaki

  Laboratory Of Animal Beproduction Faculty Of Agriculture Tohoku University

• Umezu Motoaki

  Laboratory Of Animal Endocrinology And Reproduction Faculty Of Agriculture Utsunomiya University

• MASAKI Tamotsu

  Laboratory of Animal Endocrinology and Reproduction, Faculty of Agriculture, Utsunomiya University

• Masaki Tamotsu

  Laboratory Of Animal Endocrinology And Reproduction Faculty Of Agriculture Utsunomiya University

• UMEZU Motoaki

  Laboratory of Animal Endocrinology and Reproduction, Faculty of Agriculture, Utsunomiya University

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