Cloning of Ethylene Responsive Genes from the Apices of Cucumber Plants (Cucumis sativus L. )
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概要
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The technique of differential display was used to isolate ethylene-responsive cDNAs from the apices of cucumber plants. Differences in RNA populations from apices treated with or without ethephon (an ethylene-releasing compound) were examined using 80 primer combinations. Northern blot analysis confirmed that 20 cDNAs represented mRNAs that were differentially expressed upon ethephon treatment (promoted, 17; suppressed, 3). Sequence analysis of these cDNAs revealed that two clones were identical to the 3-terminal regions of the CR20 and CUS3 genes of cucumber. Five clones showed significant similarity to the C-terminal regions of short-chain alcohol dehydrogenases, β-1, 3-glucanases, S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, products of the Aux/IAA gene family, and lipoxygenases. The other 13 clones were found to have no significant homology in the databases. Because ethylene promotes female flower formation in cucumber plants, some of 20 cDNAs might be involved in processes of sex regulation.
- 2001-06-01
著者
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SAKAI Shingo
Institute of Biological Science, University of Tsukuba
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Sato Yuka
Institute Of Biological Sciences University Of Tsukuba
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Sakai Shingo
Institute Of Biological Sciences University Of Tsukuba
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ANDO Sugihiro
Institute of Biological Sciences, University of Tsukuba
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Sakai Shingo
Institute For Biochemical Regulation Faculty Of Agriculture Nagoya University
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Ando Sugihiro
Institute Of Biological Sciences University Of Tsukuba
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Sakai Shingo
Institute of Biological Sciences, University of Tsukuba
関連論文
- Purification and Properties of an Auxin-Binding Protein from the Shoot Apex of Peach Tree
- Partial Purification of a Doluble Hibberellin-Binding Protein from Mung Bean Hypocotyls
- Isolation of Gibberellin-binding Proteins by Aflinity Chromatography
- Cloning of Ethylene Responsive Genes from the Apices of Cucumber Plants (Cucumis sativus L. )
- Expression of Two 1-Aminocyclopropane-1-Carboxylate Synthase Genes, CS-ACS1 and CS-ACS2, Correlated with Sex Phenotypes in Cucumber plants (Cucumis sativus L. )
- IDENTIFICATION AND CHARACTERIZATION OF AN ERAF17 cDNA THAT EXPRESSION AT APICES WAS CORRELATED WITH THE SEX EXPRESSION IN CUCUMBER PLANTS
- Cloning of a cDNA for a 1-Aminocyclopropane-1-Carboxylate Synthase That Is Expressed during Development of Female Flowers at the Apices of Cucumis sativus L.
- Molecular Cloning and Analysis of the cDNA for an Auxin-Regulated Calmodulin Gene : GROWTH AND DEVEROPMENT : GENE STRUCTURE AND EXPRESSION
- Auxin-induced ethylene production by mungbean hypocotyl segments
- Interaction between Auxin-binding Protein-I and RNA Polymerase II
- Properties of Gibberellin-binding Proteins from Normal and Dwarf-8 (A Dominant Gibberellin Non-responding Dwarf NIutant) Seedlings of Zea mays L.
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