Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis
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概要
- 論文の詳細を見る
The fission yeast cps6-153 mutant was originally isolated based on its hypersensitivity to the spindle poison isopropyl N-3-chlorophenyl carbamate (CIPC). The mutant also shows defects in both cell wall integrity and cytokinesis, resulting in the acccumulation of unseparated cells with weakened cell walls. The arrested cells display a disoriented alignment of cytoplasmic microtubules. When the mutant cells are cultivated at high temperature (35°C), both cell walls and septa become very thick. Electron microscopy revealed the disorganized structure of the thickened cell walls and septa, in which fibrillar components were not completely masked with an amorphous matrix. rad25+ was cloned from a genomic library by complementation of the mutant phenotypes, suggesting the involvement of Rad25p, one of two 14-3-3 proteins in S. pombe, in the pathway of cell wall integrity and cytokinesis.
- 日本遺伝学会の論文
著者
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ISHIGURO Junpei
Department of Biology, Faculty of Science and Engineering, Konan University
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Ishiguro Junpei
Department Of Biology Faculty Of Science And Engineering Konan University
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SHIMADA Shin-ichiro
Department of Biology, Faculty of Science and Engineering, Konan University
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GABRIEL Miroslav
Department of Biology, Faculty of Medicine, Masaryk University
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KOPECKA Marie
Department of Biology, Faculty of Medicine, Masaryk University
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Kopecka Marie
Department Of Biology Faculty Of Medicine Masaryk University
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Shimada Shin-ichiro
Department Of Biology Faculty Of Science And Engineering Konan University
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Gabriel Miroslav
Department Of Biology Faculty Of Medicine Masaryk University
関連論文
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- 分裂酵母におけるスピンドル毒CIPC超感受性変異株の分離と分析〔英文〕
- Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis
- 分裂酵母のスピンドル毒(CIPC)超感受性変異に関与する遺伝子の分離と分析〔英文〕
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