分裂酵母のスピンドル毒(CIPC)超感受性変異に関与する遺伝子の分離と分析〔英文〕
スポンサーリンク
概要
- 論文の詳細を見る
The cps3 gene of the fission yeast, Schizosaccharomyces pombe, was previously identified as a mutation conferring supersensitivity to the spindle poison, isopropyl N-3-chlorophenyl carbamate (CIPC). A 3.2 kb DNA fragment that complements the mutant phenotype was cloned from a S. pombe genomic library. The base sequence analysis showed that the fragment contains a maximum 1086 nucleotide open reading frame and that the putative product consists of 362 amino acids, having a molecular weight of 39.3 KDa. No significant homology of the potential product with known proteins could be found by database searches. A disruptant of the gene, produced by insertion of a ura4+ fragment was able to germinate, but not to undergo cell division, suggesting that the gene to be essential for the cell cycle progression. The disruption experiment suggests that the gene is an extragenic suppressor of cps3 mutation.
- 日本遺伝学会の論文
- 1994-12-01
著者
-
石黒 順平
Department Of Biology Faculty Of Science Konan University
-
Kawahara Kouji
Department Of Biology Faculty Of Science Konan University
-
Ishiguro Junpei
Department Of Biology Faculty Of Science And Engineering Konan University
-
Uhara Yukiko
Department Of Biology Faculty Of Science Konan University
-
右原 由紀子
Department of Biology, Faculty of Science, Konan University
-
河原 康二
Department of Biology, Faculty of Science, Konan University
関連論文
- 電気穿孔法による分裂酵母の実用的形質転換法
- Inhibitory Activity of 1-Farnesylpyridinium on the Spatial Control over the Assembly of Cell Wall Polysaccharides in Schizosaccharomyces pombe
- Abnormal formation of the glucan network from regenerating protoplasts in Schizosaccharomyces pombe cps8 actin point mutant
- 酵母サッカロミセス・セレビシエイにおけるブラスチサイジンS抵抗性遺伝子,bls2,の染色体マッピング(速報)〔英文〕
- 分裂酵母におけるスピンドル毒CIPC超感受性変異株の分離と分析〔英文〕
- Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis
- 分裂酵母のスピンドル毒(CIPC)超感受性変異に関与する遺伝子の分離と分析〔英文〕
- Characterization of a fission yeast P_5-type ATPase homologue that is essential for Ca^/Mn^ homeostasis in the absence of P_2-type ATPases
- Genetic control of fission yeast cell wall synthesis : the genes involved in wall biogenesis and their interactions in Schizosaccharomyces pombe
- Comparative electrophoretic study on proteins from mitochondrial and cytoplasmic ribosomes of Saccharomyces cerevisiae