Protective Effect of Rifampicin against Acute Liver Injury Induced by Carbon Tetrachloride in Mice
スポンサーリンク
概要
- 論文の詳細を見る
Rifampicin conferred significant protection against carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Serum alanine transaminase (ALT) and aspartate transaminase (AST) activities were not markedly altered and only hepatocellular fatty degeneration was found in mice pretreated with rifampicin (200 mg/kg), whereas severe centrilobular necrosis was observed and serum ALT and AST activities were as high as 281 and 271 I.U./1, respectively, in the control group following administration of CCl<SUB>4</SUB> (400μl/kg). The contents and activities of microsomal drug-metabolizing enzymes in rifampicin-pretreated animals were also much higher than those of the controls. CCl<SUB>4</SUB>-mediated malondialdehyde (MDA) formation was increased in rifampicin-treated liver microsomes, demonstrating that rifampicin was capable of increasing the NADPH-dependent metabolism of CCl<SUB>4</SUB> catalyzed by P-450 2E1 to produce free radicals. However, MDA formation was obviously depressed by rifampicin at varying concentrations from 2 to 32 x 10<SUP>-6</SUP> M in an in vitro cytochrome P-450 (P-450) enzyme system. On the other hand, NADPH oxidation in the metabolism of CCl<SUB>4</SUB> and aniline hydroxylation were not suppressed in the presence of rifampicin in this systems, suggesting that rifampicin did not influence the biotransformation of CCl<SUB>4</SUB> by P-450 2E1 in vitro. Therefore, the protective effect of rifampicin against CCl<SUB>4</SUB> hepatotoxicity appeared to result from the direct inhibition of lipid peroxidation generated by CCl<SUB>4</SUB>-derived free radicals.
- 社団法人 日本薬理学会の論文
- 1995-12-01
著者
-
Inoue Kyoichi
Third Department Of Internal Medicine Kansai Medical University
-
Takasu Masashi
Third Department Of Internal Medicine Kansai Medical University
-
HUANG Renbin
Third Department of Internal Medicine, Kansai Medical University
-
OKUNO Hiroyasu
Third Department of Internal Medicine, Kansai Medical University
-
SHIOZAKI Yasuko
Third Department of Internal Medicine, Kansai Medical University
-
Huang Renbin
Third Department Of Internal Medicine Kansai Medical University
-
Okuno Hiroyasu
Third Department Of Internal Medicine Kansai Medical University
-
Shiozaki Yasuko
Third Department Of Internal Medicine Kansai Medical University
関連論文
- Multidrug resistance3 is in situ detected in the liver of patients with primary biliary cirrhosis, and induced in human hepatoma cells by bezafibrate
- PANCREATIC PLEURAL EFFUSION SUCCESSFULLY TREATED BY ENDOSCOPIC PANCREATIC DUCT DRAINAGE COMBINED WITH EXTRACORPOREAL SHOCK-WAVE LITHOTRIPSY : REPORT OF A CASE
- Development of local necrosis, side-effects and histopathological study after hepatic injection of acetic acid solutions
- Hypercoagulopathy with Piperacillin Administration in Osteomyelitis
- Induction of Metallothionein Isoforms in Rat Hepatoma Cells by Various Anticancer Drugs
- Lactulose-L-rhamnose intestinal permeability test in patients with liver cirrhosis
- Influence of biliary obstruction on neutrophil chemotaxis
- Endoscopic Bilateral Hepatic Drainage with Self-Expandable Metallic Stents for Malignant Hilar Stricture
- Long-term prognosis of primary biliary cirrhosis (PBC) in Japan and analysis of the factors of stage progression in asymptomatic PBC (a-PBC)
- SOLITARY JUVENILE POLYP DEVELOPING ADENOCARCINOMA WITH SUBMUCOSAL INVASION
- Role of endogenous platelet-activating factor in the regulation of pancreatic blood flow during caerulein stimulation
- Lack of in vitro effects of interferon on drug-metabolizing activity in the human liver
- Effects of Rifampin on the Glutathione Depletion and Cytochrome c Reduction by Acetaminophen Reactive Metabolites in an In Vitro P450 Enzyme System
- Protective Effect of Rifampicin against Acute Liver Injury Induced by Carbon Tetrachloride in Mice
- Drug metabolizing activity in rats with chronic liver injury induced by carbon tetrachloride: Relationship with the content of hydroxyproline in the liver.
- Effect of malotilate (diisopropyl 1,3-dithiol-2-ylidenemalonate) on drug metabolizing activity in rat liver microsomes.
- Comparison of the effects of various inducers on 7-alkoxycoumarin O-dealkylase activities in liver microsomes.
- The effect of galactosamine on rat liver cytochrome P-450 activities.