DEVELOPMENT OF A NEW PROTEIN TRANSDUCTION SYSTEM BY FUSING N-TERMINAL PEPTIDE OF PARTIAL CHOLERA TOXIN B SUBUNIT

概要

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Cholera toxin B subunit (CTB) has been shown to be incorporated into mammalian cells and targeted to the Golgi apparatus. To examine whether partial length of CTB peptides are incorporated into the cells, we prepared recombinant partial length-CTB s (34-35 amino acids). N-terminal 34-mer peptide of partial CTB (nrCTB) bound to the cell surface and this binding ability was similar to that obtained with full length-CTB. To assess whether nrCTB can transduce a protein into the cells, a recombinant nrCTB-green fluorescence protein (EGFP) fusion protein was made. The nrCTB-EGFP was readily internalyzed into the cytoplasm. These results introduce a new protein transduction system by fusing N-terminal peptide of cholera toxin B subunit.
名古屋市立大学の論文
2003-02-28