Illumination by Near-Critical-Angle Incidence for Imaging Fluorescence Correlation Spectroscopy with Electron-Multiplying Charge-Coupled Device Camera

概要

論文の詳細を見る
We developed a novel illumination method for imaging fluorescence correlation spectroscopy (imaging FCS) with electron-multiplying charge-coupled device (EM-CCD) to regulate the depth of observation volume. In this method, a laser beam for excitation is incident to a glass-water boundary at an angle slightly smaller than critical angle. Transmitted light excites molecules only in the vicinity of the boundary. Through an FCS imaging experiment and a simulation based on a finite-difference time-domain (FDTD) method, we have confirmed that field intensity decays exponentially with distance and penetration depth varies with incident angle until 2 μm, which is more than 10 times larger than that of a typical evanescent field.
2010-06-25