Dehydration Process of Protein Crystals by Micro-Brillouin Scattering

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Polymorphism and dehydration process have been studied by the micro-Brillouin scattering technique in hen egg white lysozyme crystals without cross-linking. Two types of crystal with tetragonal and monoclinic systems have been successfully grown by the two-liquid interface method. The dehydration processes of tetragonal and monoclinic crystals have been investigated by the exposure of crystals to open air. Sound velocity increases markedly owing to the increase in intermolecular interaction between lysozyme molecules, while the attenuation of sound wave decreases markedly owing to the decrease in friction generated by mobile water. The time dependences of sound velocity in two crystals have been discussed on the basis of the Avrami–Erofe’ev model. It is found that a monoclinic crystal dehydrates much faster than a tetragonal one.
2008-05-25