Shock Wave Based Biolistic Device for DNA and Drug Delivery
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概要
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A shock wave assisted biolistic (biological ballistic) device has been developed to deliver DNA/drug-coated micro-projectiles into soft living targets. The device consists of an Nd:YAG laser, an optical setup to focus the laser beam and, a thin aluminum (Al) foil (typically 100 μm thick) which is a launch pad for the micro-projectiles. The DNA/drug-coated micro-particles to be delivered are deposited on the anterior surface of the foil and the posterior surface of the foil is ablated using the laser beam with an energy density of about $32\times 10^{9}$ W/cm2. The ablation launches a shock wave through the foil that imparts an impulse to the foil surface, due to which the deposited particles accelerate and acquire sufficient momentum to penetrate soft targets. The device has been tested for particle delivery by delivering 1 μm size tungsten particles into liver tissues of experimental rats and in vitro test models made of gelatin. The penetration depths of about 90 and 800 μm have been observed in the liver and gelatin targets, respectively. The device has been tested for in vivo DNA [encoding $\beta$-glucuronidase (GUS) gene] transfer by delivering plasmid DNA-coated, 1-μm size gold (Au) particles into onion scale, tobacco leaf and soybean seed cells. The GUS activity was detected in the onion, tobacco and soybean cells after the DNA delivery. The present device is totally non-intrusive in nature and has a potential to get miniaturized to suit the existing medical procedures for DNA and/or drug delivery.
- 2008-03-25
著者
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Nakada Mutsumi
Graduate School Of Life Sciences Tohoku University
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Takayama Kazuyoshi
Biomedical Engineering Research Organization Tohoku University
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Kanno Akira
Graduate School Of Life Sciences Tohoku Univ.
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Menezes Viren
Department of Aerospace Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400-076, India
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Hosseini S.
Department of Bioengineering, University of Washington, 1705 N.E. Pacific St., Box 355061, Seattle, WA 98195, U.S.A.
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Nakada Mutsumi
Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
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Kanno Akira
Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan
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Takayama Kazuyoshi
Biomedical Engineering Research Organization (TUBERO), Tohoku University, Sendai 980-0872, Japan
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