Accuracy Estimation in Force Spectroscopy Experiments

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概要

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• Force spectroscopy is a useful tool for the investigation of molecular interactions. We here present a detailed analysis of parameter estimation in force spectroscopy experiments. It provides the values of the statistical errors of the kinetic off-rate constant $k_{\text{off}}$ and the energy length scale $x_{\beta}$ to be considered using the single barrier model. As a biologically relevant experimental system we used the interaction between human rhinovirus serotype 2 and a recombinant derivative of the very-low density lipoprotein receptor. The interaction forces of single virus–receptor pairs were measured at different loading rates and analysed according to the single barrier model. Accuracy estimates of $k_{\text{off}}$ and $x_{\beta}$ were obtained by Monte Carlo simulation and bootstrapping. For this model of virus–receptor attachment, force spectroscopy experiments yielded $x_{\beta}=(0.38\pm 0.07)$ nm and $\ln k_{\text{off}}=(-2.3\pm 1.0)\ln$ s-1.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2007-08-30

著者

• Blaas Dieter

  Max F. Perutz Laboratories Medical University Vienna

• Gruber Hermann

  Institute for Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria

• Kienberger Ferry

  Institute for Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria

• Blaas Dieter

  Max F. Perutz Laboratories, Medical University of Vienna, Institute of Medical Biochemistry, Vienna Biocenter, Dr. Bohr Gasse 9/3, A-1030 Vienna, Austria

• Rankl Christian

  Institute for Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria

• Hinterdorfer Peter

  Institute for Biophysics, University of Linz, Altenbergerstr. 69, A-4040 Linz, Austria

関連論文

• Virus analysis by electrophoresis on a microfluidic chip
• Analysis of liposomes by capillary electrophoresis and their use as carrier in electrokinetic chromatography
• Accuracy Estimation in Force Spectroscopy Experiments

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