Design of High-resolution Analysis Technique for Capillary Electrophoresis Microchip
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概要
- 論文の詳細を見る
In this study, we develop an electrophoresis microfluidic device featuring a conventional cross-form injection system and an expansion chamber located at the inlet of the separation channel. The combined injection system/expansion chamber arrangement is designed to deliver a high-quality sample band into the separation channel such that the detection performance of the device is enhanced. The current microfluidic device is investigated using a sample of Rhodamine B dye. Also, the effects of the expansion chamber on capillary electrophoresis (CE) separation are studied by considering the separation of \textit{Hae III-digested $\phi$x-174 DNA samples}. The results indicate that an expansion chamber with an expansion ratio of 2.5 and an expansion length of 500 μm delivers a sample plug with the correct shape and orientation. The microfluidic device developed in this study has an exciting potential for use in high-performance, high-throughput chemical analysis applications and in many other applications throughout the field of micro-total-analysis systems.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-10-15
著者
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Tsai Chien-hsiung
Department Of Vehicle Engineering National Pingtung University Of Science And Technology
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Fu Lung-Ming
Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
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Leong Jik-Chang
Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
関連論文
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- Rapid Microfluidic Mixers Utilizing Dispersion Effect and Interactively Time-Pulsed Injection
- Design of High-resolution Analysis Technique for Capillary Electrophoresis Microchip
- Design of Interactively Time-Pulsed Microfluidic Mixers in Microchips using Numerical Simulation