The Effect on Cell-Adhesion of Ion-Bombardment into Collagen

概要

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A study has been conducted on the dependence of cell-adhesion on ion-bombarded collagen-coated polystyrene. Type I collagen was coated onto a polystyrene plate and bombarded with Ar+ at 50 keV at doses between $1\times 10^{13}$ and $1\times 10^{16}$ ions/cm2. Bovine aorta endothelial cells were cultured onto ion-bombarded specimens in a culture medium. The bovine endothelial cells adhered surfaces were mounted on parallel-plate flow chambers, in a flow system prepared for cell-adhesiveness tests with a flowing shear stress of 2 Pa. Adhesion strength was measured by the number of cells adhered to the specimen per 1 mm2. The cell-adhesion of Ar+-ion-bombarded specimens increased for doses up to $1\times 10^{14}$ ions/cm2, and decreased at doses above $1\times 10^{15}$ ions/cm2. IR spectra showed that the structure of the collagen was destroyed as the dose increased. The increase in cell-adhesion at the lower dose region around $1\times 10^{14}$ ions/cm2 is probably due to less destruction of collagen structure, and an increase in adhesion by the generation of new bonds around ion tracks in the interface between the collagen and polystyrene.
Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
2003-07-15