In Vitro and In Vivo Effects of Xanthorrhizol on Human Breast Cancer MCF-7 Cells Treated With Tamoxifen

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概要

• 論文の詳細を見る

• This study investigated the herb–drug interaction of xanthorrhizol and tamoxifen in human breast cancer cells. Using MCF-7 cell line as an in vitro model, the herb–drug interaction between xanthorrhizol and tamoxifen was measured by MTT assay, luciferase reporter assay, and cell cycle analysis. The effects of xanthorrhizol on growth/autophagy related signaling were determined by immunostaining, western blotting, and real time RT-PCR. Additionally, the in vivo effect of xanthorrhizol and tamoxifen on athymic nude mice implanted with MCF-7 cells was evaluated. When MCF-7 cells were co-treated with tamoxifen and xanthorrhizol, there were no significant changes in terms of cell number, luciferase activity, percentage S-phase cells and LC3-II expression. However, using the MCF-7 implanted nude mice model, it was possible to detect significantly increased tumor volumes, a larger tumor size, and increased protein expression of P38 and P27(Kip1) in the xanthorrhizol + tamoxifen group compared to the tamoxifen-alone group. It can be concluded that while there is no significant herb–drug interaction between xanthorrhizol and tamoxifen in vitro, there is such an interaction in tumor-bearing mice, which provides important information that affects breast cancer treatment translational research.

• 公益社団法人 日本薬理学会の論文

著者

• Chen Wei-shone

  Divisions Of Colorectal Surgery Veterans General Hospital-taipei And National Yang-ming University

• Chiu Jen-hwey

  Institute Of Traditional Medicine National Yang-ming University

• SHYR Yi-Ming

  Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital

• Chang Chun-Ju

  Department of Food Science, National Taiwan Ocean University, Taiwan

• Noomhorm Nattanant

  Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taiwan

• Wen Che-Sheng

  Department of Orthopedics, Cheng-Hsin General Hospital, Taiwan

• Wang Jir-You

  Department of Orthopedics, Taipei Veterans General Hospital, Taiwan

• Chen Jiun-Liang

  School of Traditional Chinese Medicine, Chang-Gung University, Taiwan

• Tseng Ling-Ming

  Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taiwan

• Chen Wei-Shone

  Division of Colorectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Taiwan

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• In Vitro and In Vivo Effects of Xanthorrhizol on Human Breast Cancer MCF-7 Cells Treated With Tamoxifen

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