Effects of S/B Remedy Containing Scutellaria baicalensis and Bupleurum scorzonerifolfium on Hepatic Interleukin-6 Related Signal Transducer and Activator of Transcription 3 Activation in Mice through Cell–Cell Interaction

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概要

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• Signal transducer and activator of transcription 3 (STAT3) plays an important role in regulating interleukin 6 (IL-6) related growth control of the liver. Our previous study demonstrated that a mixture containing Scutellaria baicalensis and Bupleurum scorzonerifolfium (S/B remedy) modulated the growth of hepatocytes during liver regeneration after 2/3 partial hepatectomy. The aim of this study was to investigate whether S/B remedy induced mouse hepatic STAT3 activation directly in hepatocytes or indirectly via non-parenchymal cell–hepatocyte interaction. Direct S/B remedy effects were studied using primarily isolated hepatocytes; while C57BL/6J mice were used to study indirect effects of S/B remedy using gadolinium chloride to deplete Kupffer cells function. The results showed that S/B remedy and its active constituents did not directly activate growth-related signaling in primarily isolated hepatocytes. However, S/B remedy induced STAT3 and subsequently suppressor of cytokine signaling (SOCS3) activation in mouse liver and increased serum IL-6 level in a dose-dependent manner, which could be partially blocked by pretreatment with gadolinium chloride. Oligonucloetide microarray analysis from S/B remedy-treated peripheral blood leukocytes demonstrated an up-regulation of IL-6 gene expression. We conclude that S/B remedy did not directly induce STAT3 activation in vitro, but induced hepatic IL-6 related STAT3 activation through non-parenchymal cell-hepatocyte interaction in vivo. The results provide important information on the molecular mechanisms of S/B remedy for treatment of human liver diseases.

著者

• Chiu Jen-hwey

  Institute Of Traditional Medicine National Yang-ming University School Of Medicine

• JIANG JENG-KAE

  Division of Colon and Rectal Surgery, Department of Surgery, National Yang-Ming University, Taipei V

• Kuo Cheng-deng

  Department Of Medical Research And Education Biophysics Laboratory Taipei Veterans General Hospital

• Lee Chang-Yin

  Institute of Traditional Medicine, School of Medicine, National Yang-Ming University

• Wang Jir-You

  Institute of Traditional Medicine, School of Medicine, National Yang-Ming University

• Chen Tzu-Chun

  Institute of Traditional Medicine, School of Medicine, National Yang-Ming University

• Peng Chi-Hao

  Institute of Traditional Medicine, School of Medicine, National Yang-Ming University

• Chang Wen-Chi

  Laboratory Animal Center, National Yang-Ming University

• Wu Chew-Wun

  Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital

• Chiu Jen-hwey

  Institute Of Traditional Medicine National Yang-ming University

• Jiang Jeng-Kae

  Division of Colorectal Surgery, Department of Surgery, Taipei Veterans General Hospital

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