Simultaneous Assay of Phenylalanine and Tyrosine

概要

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A novel enzymatic simultaneous assay of phenylalanine and tyrosine using phenylalanine-and tyrosine-decarboxylases was developed. The two enzymes were absorbed in a small piece of filter paper, dried and placed in a test tube, followed by the addition of sample solution. After the whole quantity of the sample was absorbed into the filter paper, the tube was incubated, then t-butylmethylether containing diethylamine was added to stop the reaction and to extract the reaction products, β-phenylethylamine and tyramine. Fluorescence intensities of β-pheylethylamine and tyramine in the organic phase were then measured at 278 nm and 305 nm of emission with excitation at 255 nm, and 275 nm, respectively.
Japan Society of Clinical Chemistryの論文