Chemiluminescence Enzyme Immunoassay for Thyroxin Using Glucose Oxidase and a Leuco-2', 7'-Dichlorofluorescein-Peroxidase-Hydrogen Peroxide-Bis (2, 4, 6-Trichlorophenyl) Oxalate System
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概要
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The new chemiluminescence assay of glucose oxidase (GOD) has been developed and applied to the enzyme immunoassay using GOD as the label enzyme. GOD activity can be assayed by two reaction steps in which at the first step non-fluorescent leuco-2', 7'- dichlorofluorescein is oxidized to fluorescent 2', 7'-dichlorofluorescein (DCF) by H<SUB>2</SUB>O<SUB>2</SUB> generated from glucose with the catalytic reaction of GOD (to be assayed), and then at the second step DCF gives chemiluminescence by reaction with bis (2, 4, 6-trichlorophenyl) oxalate-H<SUB>2</SUB>O<SUB>2</SUB> in acetonitrile. Detection limits of H<SUB>2</SUB>O<SUB>2</SUB> and GOD were 10<SUP>-7</SUP> mol/l and 1.0μU/ml, respectively. This method was applied to the EIA of thyroxin (T<SUB>4</SUB>) using GOD as the label. Results for T<SUB>4</SUB> by the EIA correlated well with those for serum determined by radioimmunoassay (r=0.947).
- Japan Society of Clinical Chemistryの論文
Japan Society of Clinical Chemistry | 論文
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