Radioimmunoassay of Glycochenodeoxycholic Acid in Serum Using Specific Antisera
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概要
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New hapten-[C-3]-bovine serum albumin (BSA) conjugates were prepared from the N-succinimidyl esters of glycochenodeoxycholic acid 3-hemisuccinate and 3-hemiglutarate by coupling with BSA. The specificity of anti-glycochenodeoxycholic acid antisera elicited in rabbits by immunization with these antigens was assessed by cross-reaction studies with closely related bile acids. The results showed that antisera obtained were reasonably specific, although glycochenodeoxycholic acid 3-glucuronide and 3-sulfate exhibited marked crossreactivities. Employing these specific antisera a simple radioimmuoassay procedure for the determination of glycochenodeoxycholic acid in serum was established.
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Japan Society of Clinical Chemistry | 論文
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