A Rapid and Simple Radioenzymatic Assay of Norepinephrine in Human Serum
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概要
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A rapid and sensitive single-isotope radioenzymatic assay for norepinephrine has been developed. This method is based on the conversion of norepinephrine to tritiated epinephrine by the enzyme phenylethanolamine-N-methyltransferase (PNMT) and <SUP>3</SUP>H-S-adenosylmethionine (<SUP>3</SUP>H-SAM).<BR>100 μl of untreated plasma were added directly to the reaction mixture. After incubation, labelled epinephrine was extracted with alumina and separated from <SUP>3</SUP>H-SAM by precipitation of <SUP>3</SUP>H-SAM with tungstate and adenosine. Adenosine could be substituted for S-adenosylmethionine (SAM) to coprecipitate the remaining radioactive SAM. In order to eliminate the effects of other plasma components, catecholamine-free plasma and an internal standard had to be assayed in parallel with a sample. 400 μl of plasma was sufficient for duplicate determinations of norepinephrine.<BR>This method is simple and 25 plasma samples (100 test tubes) can be processed within 4 hours.
- Japan Society of Clinical Chemistryの論文
Japan Society of Clinical Chemistry | 論文
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