Determination of Omithine Aminotmnsferase Activity by High-Performance Liquid Chromatogmphy Effect of KH<SUB>2</SUB>PO<SUB>4</SUB> on Elution of Dihydroquimzolinium Compound
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Here we report an effect of monobasic potassium phosphate (KH<SUB>2</SUB>PO<SUB>4</SUB>) on the elution of dihydroquinazolinium compound from high-performance liquid chromatography, which is useful for the determination of ornithine aminotransferase activity.<BR>A low concentration of KH<SUB>2</SUB>PO<SUB>4</SUB> remarkably affected the elution time and the pattern of dihydroquinazolinium.<BR>At the concentration between 3 and 5 mM, it was eluted as a sharp peak and separated from other peaks, otherwise it showed a broad peak or a peak which overlapped with other Deaks.
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