A Simple Fluorometric Microassay of DNA in Mononuclear Cell Preparations Using Ethidium Bromide
スポンサーリンク
概要
- 論文の詳細を見る
A simplified fluorometric microassay of DNA in mononuciear cell preparations using ethidium bromide is described.Optimal assay conditions are as follows;sodium dodecyl Sulfate (0.015%)(2ml) was added to mononuclear cells as a detergent, then ethidium bromide (8μg/ml)(2ml) was added as a fiuorescent intercalating agent.Fluorescence intensity was measured with an excitation at 538nm and an emission at 586nm.Cellular DNA are determined in the range of 1×105-4×106cells.This fluorometric assay is simpie, rapid, highly sensitive, and directly applicable to mononuclear cell preparations.
- Japan Society of Clinical Chemistryの論文
Japan Society of Clinical Chemistry | 論文
- Estimation of the Size Distribution of Lipoproteins by Convenient Gradient Gel Electrophoresis
- Characteristics of Human Erythrocytes with Artific Ally-Increased ATP and/or 2, 3-Diphosphoglycerate Contents
- Detection of Pancreatic Kallikrein on a Cellulose Acetate Membrane using Formazan Staining Procedure
- Physiological Significance of Fatty Acid ω-Oxidation in Liver
- Disposition of Digitoxin and Digoxin in Experimental Cholestasis and Renal Failure