Fluorescent Determination of Creatine Phosphokinase Isoenzymes by Using Stream-Switching System
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概要
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A highly sensitive fluorescent determination for CPK isoenzyme with continuous flow injection system is described.<BR>In the system, -iquid chromatography with stepwise gradient and stream switching technique with six-port valve are utilized. CPK isoenzyme is separated on a micro column packed with DEAE-sepharose and NADH is produced with a coupled enzymaticreactions.Serum and reagents backgrounds are separated from fluorescence NADH by stream switching technique. A major advantage of the detection system is the sensitive monitoring of the isoenzyme activities.<BR>In the measurement of control serum, coefficient of variation was2.5%for CPK-MM, 6.6%for CPK-MB and 9.3%for CPK-BB (n=10).<BR>We have applied this technique to the patient serum to study about clinical investigation. especially CPK MB and BB activities in the case of myocardial infarction for recent ll months.
- Japan Society of Clinical Chemistryの論文
Japan Society of Clinical Chemistry | 論文
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