新規がん抗原 GKAP1 の同定と口腔扁平上皮癌での発現解析

概要

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Cancer/testis （CT） antigens are considered promising target molecules for immunotherapy for patients with various cancers.To identify CT antigens or CT-like antigens, we performed serological identification of antigens by recombinant expression cloning （SEREX） analysis that screened a testicular cDNA library with serum obtained from a patient with gastric adenocarcinoma. We isolated 4 genes with testis specific profiles in the database, including G kinase anchoring protein 1 （GKAP1）. RT-PCR analysis showed that the mRNA expression levels of GKAP1 were restricted to the testis in normal adult tissues. In malignant tissues, GKAP1 was aberrantly expressed in a variety of cancers, particularly oral squamous cell carcinoma （OSCC）. mRNA expression analysis of other CT antigens revealed that MAGE-A4 was expressed in 32％, MAGE-A3 in 24％, MAGE-A1 in 13％, CCDC62-2 in 12％, SSX-2 in 11％, XAGE-1b in 7 ％, and NY-ESO-1 in 3 ％ of OSCC. 52.5％ of OSCC expressed at least one of these antigens. Of 15 sera from cancer patients with OSCC, 2 sera were reactive with GKAP1 protein on ELISA analysis. None of the healthy donor serum was reactive.These findings suggested that GKAP1 is immunogenic in OSCC and demonstrated its potential as a diagnostic marker for patients with OSCC in combination with other CT antigens such as MAGE-A4, MAGE-A3, and CCDC62-2.
Japanese Society of Oral and Maxillofacial Surgeonsの論文