Immature Embryo Culture and Interspecific Hybridization between Capsicum annuum L. and C. frutescens L. via Embryo Rescue
スポンサーリンク
概要
- 論文の詳細を見る
The effects of the medum composition and embryo age of various cultivars on plant recovery for successful rescue of hybrid embryos from<I>Capsicum annuum</I>and <I>C. frutescens</I>were studied. Medium M<SUB>2</SUB>, a modification of Murashige and Skoog (MS) medium, supplemented with casein hydrolysate (CH) and yeast extract (YE) 500 mgl<SUP>-1</SUP>each, coconut water (CW) 150 mll<SUP>-1</SUP>, gibberellic acid (GA<SUB>3</SUB>) 0.5 mgl<SUP>-1</SUP>and naphthaleneacetic acid (NAA) 0.05 mgl<SUP>-1</SUP>, was found to be the most suitable medium for plant recovery. The supply of CH combined with sucrose exerted a beneficial effect on plant recovery and CW enhanced the effect. Hybrid plants were produced only from embryos at 28 to 33 days after pollination. Plant recovery rate from hybrid embryos was low and less than the embryo germination rate presumably due to the existence of some post-zygotic incompatible barriers. Hybridity of the recovered plants was confirmed using random amplified polymorphic DNA (RAPD) markers and based on their morphological characteristics derived from the pollen parent. Pollen fertility was very low in the hybrid plants compared with that in their parents. The simple method of embryo rescue proposed in this study could be used for the production of interspecific hybrids in<I>Capsicum</I> spp.
- 日本熱帯農業学会の論文
日本熱帯農業学会 | 論文
- 貯蔵中のランブータン果実の細胞壁代謝に及ぼす低温障害の影響
- 海南島での永年作物への被陰樹
- 台湾におけるカイラン在来品種群のアイソザイム多型性
- 近赤外分光分析法によるサトウキビ品質評価
- 41. マンゴーの花芽分化に及ぼす2、3の処理の影響について