A comparison of ototoxicity between two anticancer platinum drugs using organ culture.

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The ototoxicity of Cisplatin (CDDP) and DWA2114R (DWA) was evaluated using an organ culture system. The inner ears of mouse 21-day embryos were cultured for 4days with or without CDDP or DWA, with doses from 0.1 to 10 μg/ml. The damage of crista ampullaris of cultured inner ear was estimated accordihg to the ototoxicity score based on morphological change by a light microscopic observation of serial sections of the samples. Using this system we showed that the effect of both CDDP and DWA was obvious at a concentration of 0.1 μg/ml and that DWA showed almost the same ototoxicity as CDDP at the same concentration. It can be elucidated that in vivo ototoxicity of CDDP is more toxic than that of DWA since CDDP remains in the body fluids for longer time than DWA. The organ culture system revealed the same ototoxic potentiality of DWA as CDDP. Therefore, this result indicates that when DWA is clinically used for patients with renal dysfunction, it should be as ototoxic as CDDP.
日本耳科学会の論文