Prostaglandin synthesis by the cochlea.

概要

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This study described the exogeneous and endogeneous synthesis of prostaglandins (PGs) by the cochlea in vitro. 1) After incubation of the whole membraneous cochlea with 3H-arachidonic acid, synhthesis of various kinds of PGs was evidenced. Although PGF2α was the most abundantly synthesized PG than others, the rate of conversion from arachidonic acid to it was calculated to be only 0.03%. The synthesis of PGD2, PGE2, 6ketoF1α and TXB2 were also evidensed in this order. 2) The whole membraneous cochlea was incubated at 37°C for 0-15 minutes and PG formed were determined by radioimmunoassay. The main PG produced was PGE2, and PGD2, PGF2α, PGI2 and TXA2 were also found in the cochlea. At incubation for 0 minutes without incubation PGs were already formed to considerable level and the maximal level was obtained at 5 minutes incubation. Thereafter the levels of PGD2 PGE2 and PGF2α decreased gradually. 3) The cochlea was dissected into three parts: OC+M (organ of Corti +modiolus), LW (lateral wall) and CN (cochlear nerve), and PGs formed by these tissues was determined. In OC+M and CN main PG was PGE, whereas in LW the amount of PGD2, PGE2, PGF2α and PGI2 was about same level. These result revealed that the cochlea tissues can also prodused main five PGs as other organs. Possible involvement of PGs in various cochlear functions was briefly discussed.
日本耳科学会の論文