Preliminary report of observation on secondary degeneration of auditory nerve in KM treated guinea pigs - Quantitative analysis.:Quantitative Analysis

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Authors performed to clarify the secondary degeneration of auditory nerve in guinea pigs after the treatment of KM 200mg/kg/body weight for 28 days. First the auditory nerve of each animals was removed, and then it was immersed in fixative solution and embedded in epon by routine method. The thin cut transverse surfacespecimen of auditory nerve (0.3μm) was obtained and stained with Toluidine blue solution. Specimens, magnified to 50 times each and all, were photographed. These pictures were used for counting nerve fibers with the image analyzer (LUZEX 500, Toyo Inc.) (Fig. -1). As the results in this moment, degeneration of nerve fibers due to KM after being bred for 6 months appeared in three parts, i. e. nerve of cochlear axis (about 2.0%), nerve at near portion of Scarpa's ganglion (about 10%), and nerve at near portion of Glial-Schwann's junction (about 35%), shown in Table-1. Authors certified the deformation of auditory hairs of cells (Fig. -4, 5) and the decrease of spiral ganglion cells (Fig. -6).
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