Histochemical Study on Alteration in Energy Metabolism of Hair Cells of The Spiral Organ in Guinea Pigs Treated With Aminoglycoside Antibiotic and Later With Furosemide

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Supravital reduction reaction of Nitro-BT for demonstration of reduction potency in electron transfer system of mitochondria was performed by intracochlear perfusion in guinea pigs treated wtih kanamycin (KM, 400mg/kg, i. m.) and 2 hr. later with furosemide (100mg/kg, i. v.). Differential pinna reflex test in wide frequency range (20k-500 Hz) was performed on 20 young adult guinea pigs. Marked swelling, intracellular edema and decrease in the reaction product occurred extensively in the outer hair cells (OHC) in guinea pigs with complete loss of the pinna reflex 5 min. after the furosemide injection. These changes of OHC became decreased 2 hr. later, when the pinna reflex showed transient improvement. However, these changes became again increased 24 hr. after the injection, when the loss of the pinna reflex recurred. On 7 days after the injection, loss of OHC in the lower turn occurred.
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