THE EXPERIMENTAL STUDIES ON DISTRIBUTION OF P32-LABELED BACTERIA IN RATS
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概要
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1) P32-labeled strept. viridans or strep. hemolyticus were injected intravenously in the normal rats and rats that were sensitized with dead strep. viridans or hemolyticus, rabbit serum sensitized with heart of rat or rabbit serum sensitized with kidney of rat. The rats were sacrificed 1.5, 3 and 6 hours after injection of P32-labeled bacteria. Then, the distribution of bacteria in organs (heart, lung, liver, spleen, kidney, bone and blood) of rats was compared with radioactivity of bacteria, and colonies of bacteria in mixed culture with goat's blood.The results showed a tendency that the distribution of strep. viridans is more marked in the lesser circulatory system than in the greater circulatory system, and that of hemolyticus showed more marked in the spleen and the kidney The highest distribution of bacteria was noted inthe liver.Influence of sensitization on the distribution of bacteria was not very distinct2) The injected bacteria give off radioactive phosphorus after 6 hours in the rat body. Therefore, the radioactivity of tissue after 6 hours will not be same with the distribution of bacteria.3) The investigation of the distribution of bacteria with method of mixed culture requires skill and expert technique. Therefore, in the experimental studies on the distribution of bacteria, it is advantageous to employ the method linked with radioisotope-labeled bacteria in order to obtain a quick vesult.
- 一般社団法人 国立医療学会の論文
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