Assay of serum acid phosphatase activity with use of automatic chemical analyzer employing DCAP-P as substrate.
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Serum acid phosphatase (ACP) is known as an useful marker for diagnosis of metastatic carcinoma of the prostate. However, measurement of ACP requirs prompt treatment of the samples due to unstable and low activity levels in serum. Therefore the analysis of serum ACP has been one of the most difficult biochemical tests for automatic analysis. We evaluated an automated procedure for the assay of serum ACP activity employing 2, 6-dichloro-4-acetylphenylphosphate as substrate by Hitachi 7150.Linearity was confirmed up to 1344IU/l by dilution of elevated patient's specimen. Within-run and day to day precision (CV) were 2.0 and 3.7%, respectively. No interference from bilirubin, or turbidity was observed. But high concentration of hemolysis showed positive error for ACP activity. Relation between serum ACP activity as determined manually by the Bessey-Lowry's method (x) and current method (y) yielded the following equation. y=0.95x-0.66, r=0.966.The proposed method has advantages for the accurate, precise and rapid assay on the automated analysis with use of analyzer, Hitachi 7150.
- 一般社団法人 国立医療学会の論文
一般社団法人 国立医療学会 | 論文
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