Quantitative determination of mitomycin C in biological materials by high-performance liquid chromatography and pharmacokinetics of mitomycin C in cancer patients.

概要

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A high-peformance liquid chromatographic method was developed for the determination of mitomycin C in the biological materials, with a sensitivity of 10ng/ml. It is considered that our method is more favorable for the determination, compared to the methods already reported, giving a more clear chromatogram with a simple technique.The stability test of mitomycin C was investigated under various conditions. As a result, it was demonstrated that mitomycin C was stable in Tris-HCL buffer (pH 7.4), plasma and ascites under atmosphere at a temperature of 37°C. Mitomycin C was also stable in the 10, 000g supernatant of the homogenates of human stomach tissue, stomach cancer tissue, and human liver tissue at a concentration of 20μg/ml under atmosphere at a temperature of 37°C. Further investigations are needed at a clinical level of mitomycin C.With regard to the clinical use of mitomycin C, this drug was maintained at considerably higher concentrations over 4 hours in the ascites of the patient with peritonitis carcinomatosa, when it was administered intraperioneally, suggesting a preferable use of mitomycin C via intraperitoneal route for the treatment of peritonitis carcinomatosa.
一般社団法人国立医療学会の論文