Development of a Novel Bioassay for the Toxicity Evaluation of Suspended Particulate Matter (SPM) using Air-Liquid Interface Culture of a Human Alveolar Cell Line.

概要

論文の詳細を見る
Currently, the impact of suspended particulate matter (SPM) on humans has been concerned, but it still has many uncertainties. In vitro cytotoxicity tests using cultured cells have recently been receiving much attention, but there is few research about the evaluation of atmospheric environment. In this article, we investigated the feasibility of a new bioassay using air-liquid interface culture (ALIC) of human alveolar cells, A549, in the toxicity evaluation of SPM. In this bioassay, it was possible to mimic the in vivolike direct exposure of SPM. Carbon absorbing l-nitropyrene (l-NP), involved in the diesel exhaust gases, was loaded directly to the layer of A549 cells in ALIC culture. Dose-response relationships between the loaded amount of carbons or absorbed l-NP and cell survivability were successfully obtained. In addition, net intake in humans was evaluated by the measurement of the leaking kinetics of l-NP into the culture medium in the basolateral side. Although this assay still needs improvement, it is promising as a bioassay suitable for evaluating the toxicity of SPM samples, because not only it exactly mimics the in vivo situation but also it gives basic information about the permeation and intake of chemicals eluted from SPM deposited on the alveolar epithelia.
社団法人環境科学会の論文