Survival and Effect of Genetically Engineered Pseudomonads in the Soil Environment.
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We constructed a recombinant plasmid pSR 134 conferring mercury resistance as a sensitive marker, and introduced it into Pseudomonas putida strains PpY 101 and PRS 2000, Pseudomonas fluorescens LB 303, and Pseudomonas aeruginosa PA01 to make genetically engineered microorganisms (GEMS). We determined the applicability of the combination of mercury resistance and tetracycline resistance as a marker of microorganisms in the environment. A top agar method was developed, which enabled us to treat a large number of environmental samples to count GEMs, using a small quantity of mercuric chloride. This marker was effective for monitoring various pseudomonads in the environment. The survival of the GEMs depended on the host strains. The addition of GEMs to soil had no apparent effect on the density of indigenous microorganisms.
- 社団法人 環境科学会の論文
社団法人 環境科学会 | 論文
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