Effect of concentration of sodium laurylsulfate on motility and acrosome morphology of frozen boar spermatozoa.
スポンサーリンク
概要
- 論文の詳細を見る
The effect of concentration was examined of sodium laurylsulfate (SLS) in the initial and/or final diluents on the motility and acrosome morphology of frozen boar spermatozoa. The sperm-rich fraction collected from Landrace boars was centrifuged at room temperature and resulting sperm pellet was resuspended in a Tris-citric acid-glucose (TCG) solution (10×10<SUP>8</SUP> cells/ml). The sperm suspension was diluted 1:4 with the intitial diluent, TCG solution supplemented with egg yolk (10-30% v/v) and SLS (0-4 mg/ml), and further diluted 1: 1 with the final diluent containing 2% (v/v) glycerol after cooling to 4°C. Sperm samples were frozen on dry ice by the pellet method. The pellets were thawed at 40°C in dry test tubes. The post-thaw percentages of normal acrosomes and progressively motile spermatozoa, and the percentage of the latter after 3-hr post-thaw incubation at 37°C were improved by the addition of SLS. It was suggested that addition of SLS might be made at the initial and final dilutions, or at the final rather than the initial. The optimum concentration of SLS in the diluent containing 20% (v/v) egg yolk was considered to be 1.2 mg/ml when added at the both dilutions, and 2.0-2.8 mg/ml when added only at the final.
- 日本繁殖生物学会の論文
日本繁殖生物学会 | 論文
- 近交系Hatanoラットに認められた春機発動時期および血漿中ホルモン濃度の系統差
- ラット卵子の自発的第一卵割に及ぼすCa2+/Mg2+不含培養液の影響
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- ブタ生体回収由来ガラス化胚の生存性の改善
- 卵細胞質内注入法を用いた牛の核移植