Ovarian function and fertility after timed insemination following the treatment with PGF2.ALPHA. and LH-RH analogue in the cow.
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The present studies were conducted to clarify the effective treatment for cows with unobserved estrus. In Exp. 1, 12 cows which had received 15 mg of PGF<SUB>2α</SUB> on Day 12 after ovulation were divided into four groups of 3. They were injected with 200, 100, 50 and 25μg of LH-RH-A for Groups I, II, III and IV, respectively, 54 hrs after PGF<SUB>2α</SUB> and inseminated at 24 hrs after LH-RH-A. In Exp. 2, 15 cows which had palpable corpus luteum (CL) were divided into three groups of 5. They were injected with 200, 100 and 50μg of LH-RH-A for Groups I, II and III, respectively, after PGF<SUB>2α</SUB> and inseminated on the same time schedule as in Exp. 1. Ten control cows were in-seminated during spontaneous estrus. Ovulation was induced by 2 days after LH-RH-A and CL was formed in the cows of all groups. The LH levels at 2 hrs after LH-RH-A tended to increase in proportion to the dosage. The progesterone (P) levels in Exp. 1 in-creased gradually for 8-9 days after ovulation in the cows of Groups I and II as compared with that of Groups III and IV. The mean P level of Exp. 2 on Day 13 after ovulation was significantly (P<0.05) lower in Group I than in control. By the timed insemination, 1 cow of each group in Exp. 1, and 1 cow of Group I, 3 cows of Groups II and III, 6 cows of controls in Exp. 2 had conceived. These results indicate that the present treatments may be applicable to cows with unobserved estrus, and the effective dose of LH-RH-A is 25 or 50μg.
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