Effects of semen concentration by prefreezing centrifugation on the characteristics of frozen-thawed ram semen.

概要

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Semen samples were concentrated by centrifugation and then frozen. Effects of this pre-freezing centrifugation on the quality (motility, live sperm rate, and abnormal sperm rate) of thawed semen were examined afterwards.<BR>1. The concentrating procedure before freezing semen improved the motility and the live sperm rate, while lowered the abnormmal sperm rate.<BR>2. Similar results were obtained between the samles centrifuged immediately after the dilution and after the cooling equilibration.<BR>Changes in centrifugation speed did not affect the live sperm rate and abnormal sperm rate. But the motility was improved and the sperm concentration was increased by accelerating the speed of centrifugation.<BR>3. Increase in the proportion of the supernatant removed resulted in decrease in the motility and live sperm rate and increased in the abnormal sperm rate. Addition of the thawing solution improved the semen quatity. These effects were expressed more remarkably as sperm concentration increased. Optimum dilution rate at the thawing was not changed by different proportions of supernatant removed. The motility and live sperm rate were improved by increasing the proportion of supernatant removed when the semem was optimum diluted at thawing.
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