Number of follicles and serum levels of steroid hormone in adult rats which mated following treatment with various doses of PMSG and hCG.
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Present study was carried out to determine the factors causing poor implantation rates in adult rats treated with high doses of PMSG and hCG. Two handred ninety two rats were housed under artificial lighting (light from 07:00 to 21:00 h), and injected with 10, 20, or 40 IU of PMSG at 11:00 h on the day of early diestrus. Then the females were injected with the same dose of hCG 54h later (corresponding to the time of LH surge of normal proestrus), and mated with a male overnight. Both ovaries were removed from 24 to 120h after hCG injection (or 12 to 108 h after mating) at 24h inter vals, and were sectioned. Healthy and atretic follicles larger than 250μm in diameter were classified in each sizes of 50μm. For radioimmunoassay of progesterone, estrone and estradio1-17β, blood was collected from the abdominal aorta at the time of hCG injection and various times after hCG.<BR>1) In untreated rats, the number of healthy follicles larger than 500μm in diameter increased with time after mating. In 40 IU-treated rats at 24 and 48 h after hCG injection, the number of healthy follicles of this sizes was significantly more than that of untreated rats (<I>P</I><0.01). 2) The serum concentration of progesterone in untreated, 10 and 20 IU-treated rats increased gradually until 96 or 120 h after hCG injection, whereas in 40 IU-treated rats, progesterone level reached a peak at 72h after hCG injection, then decreased rapidly to control level by 120h. 3) At the time of hCG injection, estradio1-17β value was high in all groups, and decreased rapidly 24 h later. The low levels were maintained in untreated and 10 IU-treated groups until 120h after hCG injection. But in 40 IU-treated rats, estradiol-17β level was significantly higher than that of untreated rats at 72 h after hCG injection (<I>P</I><0.01).<BR>These results suggest that the low rates of implantation in adult rats treated with high doses of PMSG and hCG may be due to the abnormal hormonal environment in the oviducts and uterus. It may result from the high level of estradiol-17β at 72h after hCG injection and dramatic decrease in the concentration of progesterone from 96 to 120h.
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