Studies on the egg-yolk-coagulating factor in goat semen:III. Release of some acids accompanied by the coagulation phenomena
スポンサーリンク
概要
- 論文の詳細を見る
We have reported that goat semen diluted with egg yolk citrate buffer solution coagulates in several days of preservation at 4°C, and a sudden drop of pH accompanied the coagulation regardless with lactic acid accumulation. And it is probable that the coagulating factor acts to the egg yolk constituents such as phospholipids, and releases some acids which cause the drop of pH. In this experiment, we attempted to examine the occurrence of phosphorylation and the fatty acids release in the reaction mixture of egg yolk buffer solution and the coagulating factor. Principal results obtained are as follows.<BR>1. It was found that the acid soluble phosphorus was released partially from the phospholipids of the egg yolk only when the seminal plasma was used as a coagulating factor, and not when the Cowper's gland extract was used. Considering these facts, it is likely that phosphorylation is not necessarily accompanied by the coagulation phenomenon, but may be due to other enzymes present in seminal plasma of goat semen.<BR>2. There was a marked increase of fatty acids which may have been released from the egg yolk, when either the Cowper's gland extract or the seminal plasma was used as a coagulating factor, and the former has an about 10 times stronger activity in the releasing action than the latter.<BR>3) There was also an increase of neutral fats in the coagulated egg yolk buffer solution due to the released fatty acids.
- 日本繁殖生物学会の論文
日本繁殖生物学会 | 論文
- 近交系Hatanoラットに認められた春機発動時期および血漿中ホルモン濃度の系統差
- ラット卵子の自発的第一卵割に及ぼすCa2+/Mg2+不含培養液の影響
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- ブタ生体回収由来ガラス化胚の生存性の改善
- 卵細胞質内注入法を用いた牛の核移植