The effect of clomiphene on serum LH levels in immature rats primed with PMS.

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Previous reports presented that the single injection of clomiphen which is known as an antiestrogen blocks PMS-indubed ovulation in immature rats, showing 24 hours delay of ovulation. The present experiment was desinged to determine the pattern of serum LH in these rats in order to clarify the time of the LH surge for ovulation. PMS (3 IU) was subcutaneously injected to female Wistar rats at 10:00 AM on 2426 days of age. Clomiphene (Clomiphene-citrate : 2.5 mg/100 g b.w. in 30% ethanol-saline solution) was subcutaneously injected 6 hrs after PMS. Blood was collected by decapitation under ether anesthesia at various times as shown in Table 1. At autopsy ovarian and uterine weights were recorded and oviducts were examined for ova under the dissecting microscopy. Serum LH levels were measured by radioimmunoassay. Resuts are as follows: 1) Ovulation rates were 5/5 in PMS-treated group (control), and 0/5 in clomiphene-treated group, 72 hrs after PMS. But 5 out of 6 treated with clomiphen ovulated 96 hrs after PMS. 2) After 48 hrs of PMS injection significant decreases of uterine weights were observed in clomiphene treated groups as compared with PMS-treated controls (P <0, 01). 3) Highly elevated levels of serum LH were found during 5462 hrs in PMS-treated controls and during 7879 hrs in the clomiphene group, indicating 24 hours delay of LH discharge in the latter. The anti-estrogen drug may affect estrogen-receptors in the CNS-pituitary axis responsible for the LH surge on the second day-afternoon in these immature rats. The authors acknowledge the gift of rat LH radioimmunoassay materials from the Rat Pituitary Hormone Distribution Program of the National Institute of Arthritis, Metabolism and Digestive Diseases.
日本繁殖生物学会の論文

The effect of clomiphene on serum LH levels in immature rats primed with PMS.

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日本繁殖生物学会 | 論文

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