Effects of gonadotrophins on maturation and ovulation of oocytes in adult rats:I. Effect of various doses on ovulation
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概要
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Various doses (0 to 50 i.u.) of gonadotrophins were examined for effect on ovulation in adult rats. A total of 600 rats were used in the schedule of experiment, as shown in Fig. 1.<BR>The results obtained are summarized as follows.<BR>1. When rats pretreated with 50, 30, 20, or 10 i.u. of pregnant mare serum gonadotrophin (PMS) were injected with same dose of human chorionic gonadotrophin (HCG) 20 hours prior to sacrifice, the maximum number of ova ovulated by them was 43.9, 31.2, 21.8, or 16.3 per rat, respectively. When rats were killed after treatment with 50, 30, 20, or 10 i.u. of PMS alone, it was found that the average number of ova ovulated (by the number of rats ovulated in parentheses) was 1.0 (1/10), 2.0 (3/10), 6.5 (4/10), or 12.1 (9/10), respectively.<BR>2. The percentage of ovulated rats and the number of ovulated ova were reduced with a decrease in the dose of HCG. This tendency was notable when rats had been treated with a lager dose of PMS and a small dose of HCG (below 1/2 or 1/3 of the dose of PMS).<BR>3. Superovulation-treated rats showed a complete ovulation a few hours later than untreated control rats. The percentage of ovulated rats and the number of ovulated ova, however, increased with the lapse of time after HCG treatment. A complete ovulation was delayed a little in rats treated with a small dose of PMS.<BR>4. The results mentioned above suggest that it may be necessary for the induction of superovulation in adult rats to inject with such dose of HCG as corresponding to about 1/2 (or least 1/3) of the dose of PMS.
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