Adequate pre-freezing time in the frozen preservation of poultry spermatozoa
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概要
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The present experiment was planned to determine an adequate pre-freezing time for froz-en preservation of drake spermatozoa. Five pre-freezing times of 0, 1, 2, 3 and 5 minutes were set up. The relationship between each pre-freezing time and temperature change in the semen sample was tested by chromel-alumel thermocouple. At the same time effect of the pre-freezing on the sperm motility was examined after freezing and thawing.<BR>Marked difference was found in the change of the semen temperature according to the times of pre-freezing. The temperature of semen sample have shown a sharp fall to -196°C in about 20 seconds when it was exposed to 0 minute pre-freezing. When the samples were exposed to 1, 2, 3 and 5 minutes pre-freezing, each temperature went down about to -11°C -94°C, -149°C and -180°C slowly and when these samples were preserved in liquid nitrogen, the temperature dropped suddenly to -196°C. The sperm motility of these semen samples after thawing were 9, 58, 79, 82 and 80% (over ?? ) in 0, 1, 2, 3 and 5 minutes pre-freezing, resp-ectively.<BR>From these results, it seems that the adequate pre-freezing time in the freezing process of drake spermatozoa is 2 or 3 minutes and it needs not to be over 5 minutes.
- 日本繁殖生物学会の論文
日本繁殖生物学会 | 論文
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