The effect of estrogen and progestin on clomiphene blocked ovulation in PMS-primed immature rats
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The effects of estrogen and progestin on clomiphene blocked ovulation were studied in PMS-clomiphene treated immature rats.<BR>The animals were injected with 3 IU PMS subcutaneously at 9:3010:00 A. M. during 2426 days of age. Clomiphene (clomiphene citrate: 2.5 mg per 100 g. b. w. in 30% ethanol-saline solution) was injected subcutaneously 6 hr after PMS treatment.<BR>Animals were killed 74 hr after PMS injection to examine the oviducts for ova, and ovarian and uterine weights were measured.<BR>Results obtaind were as follows:<BR>1) Ovulation was inhibited on the 3rd day morning after PMS-clomiphene treatment; ovulation delayed 24 hr by the treatment. No effect on ovulation was observed by hysterectomy (Table 1).<BR>2) Estradiol 17-β (0.05200 μg per 100 g, b. w. in sesame oil) was singly injected at various times after PMS in PMS-clomiphene treated rats. High rate of ovulation was obtained by the treatment with 0.5 μg (4/6) and 5 μg (5/6) at 3 hrs, 5 μg (3/6) at 6 hrs, 5 μg (6/6) at 9 hrs, or 5 μg (5/6) at 51 hrs. It is suggested that estradiol can compete with clomiphene on the the receptor (s) during 3 to 9 hrs and it can stimulate release of ovulating hormone at 51 hrs after PMS injection (Table 2).<BR>3) Progesterone (0.022 mg per 100 g b. w. in sesame oil) was injected in the same way as estradiol.<BR>Higher ovulation rate was found in the treatment of 0.2 mg (3/4), 2 mg (3/5) at 45 hrs, 0.2 mg (2/2), 2 mg (6/6) at 48 hrs, 0.2 mg (5/6), 2 mg (5/5) at 51 hrs, and 0.2 mg (3/5), 2 mg (4/4) at 54 hrs. It is suggested that progesterone can stimulate the release of ovulating hormone during 45 to 54 hrs after PMS on the day prior to ovulation (Table 3).<BR>4) When other steroids by single treatment with cortisone acetate, corticosterone, or 17 α-OH-progesterone was injected at 51 hrs after PMS injection in PMS-clomiphene treated rats, no ovulation occurred (Table 4).
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