Studies on uterine secretion of cows:V. Someorganic constituents of uterine washings in normal and infertile cows
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Fifty five bloodless uterine washings were collected from 22 normal and 25 infertile lactating Holstein-Friesian cows.<BR>Uterine washings were obtained using an endotracheal tube with a polyethylene bac placed in the uterus per vaginam. The uterine cavity was flushed repeatedly three times with 100 ml phy-siological saline. The washings were stored in an ice box.<BR>The recovery rate of flushing fluid used to obtain uterine washings was 50 % in average (4070 % in range).<BR>The lipid content was estimated by the silver dichromate method of Bloor as modified by Katsura-Hatakeyama, toatal-N was determined by the micro-Kjeldahl procedure and reducing sugar by the colourimetric technique of somogyi, glycogen by the anthrone method of Good and Morris as modified by Kanemaki<SUP>14)</SUP>.<BR>The results obtained were as follows.<BR>1. In normal group, the levels of total lipid, total-N and reducing sugar in uterine washings showed a definite variation with stage of estrous cycle. Each amount of three constituents was lowest at estrus and increased gradually during the luteal stage. The values within 5 days to 15 18 days postestrus were maintained significantly higher than that at estrus.<BR>The ratios of the constituent levels at 1518 days postestrus to those at estrus were 2.8 in total lipid, 3.9 in total-N and 5.1 in reducing sugar, respectively. Glycogen was detected little in the washings.<BR>2. In the groups of acute and chronic-catarrhal endometoritis, the values of three constituents in uterine washings recovered at mid-luteal stage (10 to 15•8 days postestrus) were lower than those at the same stage of cycle in the normal group, and significant difference appeared in total lipid and total-N levels of both groups.<BR>3. Each mean value of the components in follicular cyst and lutein cyst groups was situated between the values at estrus and mid-luteal stage of cycle. Total lipid and total-N levels in lutein cyst group were significantly lower than those at the same stage (10 days postestrus) in the normal group.<BR>4. Levels of the three constituents in ovarian atrophy group were lowest among the five in-fertile groups, and the values of total lipid and total-N were significantly lower than those at estrus in the normal group.
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