Immunological Studies on Gonadotrophins in Animal.:VI. Cross-reaction of animal gonadotrophins with antiserum to human chorionic gonadotrophin.

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Immunological cross-reactivity of anterior pituitary gonadotrophin of bovine, equine, ovine and porcine origin and pregnant mare serume gonadotrophin (PMSG) with antiserum to human chorionic gonadotrophin (HCG) was studied by the methods of agar gel double diffusion and hemagglutination inhibition reaction. Hemagglutination inhibition reaction was examined by a reagent of commercial pregnancy test. None of the anterior pituitary total gonadotrophin preparations of bovine, equine, ovine and porcine origins and PMSG developed precipitin line against antiserum to HCG absorbed with urine of child in agar gel double diffusion plate. Purified gonadotrophins, luteinizing hormone (LH) of bovine, equine and ovine origins and follicle stimulating hormone (FSH) of ovine porcine origins, also developed no precipitin lines against antiserum to HCG absorbed with urine of child in agar gel double diffusion plate. All of these gonadotrophic hormone preparations did not inhibit the hemagglutination reaction of HCG sensitized blood cells and antiserum to HCG. These results indicate nonexistence of cross-reactivity between gonadotrophins of various species of animals and antiserum to HCG. It seems, therefore, antiserum to HCG in not applicable for the immunoassay of animal gonadotrophins.
日本繁殖生物学会の論文