Free amino acids in the uterine fluid of rats
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Studies on metabolism of amino acid and synthesis of protein in rat embryo have done in our laboratory <SUP>13)</SUP>. Thus, it is necessary to investigate the relationship between incorporation rate in embryo and content of free amino acids in the uterine fluid under various conditions. <BR>In this paper, free amino acids in the rat uterine fluid in sexual cycle and under various hormonal. conditions are reported.<BR>Adult female rats of Wistar Strain weighing from 180 to 250 g were used. Uterine fluids were collected from seven groups as follows: Group I-Natural proestrus, killed on 2 P.M., Group II-Natural estrus, killed on 9 P.m., Group III-The cervix was ligated through 2-cycle, Group IV-Pseudopregnancy, Group V-Castration and estrone injection (4 μg/day), Group VI-Castration and pro-gesterone injection (4 mg/day) and Group VII-Castration, estrone (1 μg/day) and progesterone (4 mg/day) injection. Each group consists of 24 rats. The schedules for the experiment are shown in Fig. 1. The accumulated uterine fluids were aspirated with a sterilized syringe. When the uterine fluids were-so little, the uterus were washed repeatedly with 0.2 m<I>l</I> of sterilized physiological saline and then. the washed fluids were aspirated. The aspirated fluid was recorded the volume immediately (Table 1). The fluids collected were centrifuged and stored at -20°C until the measurement. These fluids.. were deproteinized with ethanol (final conc. 75%) and then were centrifuged and the supernatant were distillated under diminished pressure. The amino acid was determined with amino acid auto-analyzer. In Group IV, the measurement was divided to two subgroups : the accumlated fluid and washed fluid. <BR>The amount of each uterine fluid is in agreement with the results<SUP>13, 14)</SUP> reported previously. The sixteen amino acids of uterine fluid were identified in all groups (Table 2). The content of glutamic acid was the highest value in all group. On the other hand, it has reported that glycine was the highest amino acid in rabbit. Contents of glutamic acid, alanine, threonine and glycine was similar in group IIII. The increase in the contents of methionine and threonine was observed in pseudopregnancy (group IV). These amino acids seemed to be important for blastocyst development<SUP>2022)</SUP>. The results of these experiments demonstrate the effect of hormonal status of the animal on theuterine secretions. Threonine, alanine, argine, glutamic acid, leucine, serine and other amino acids have increased in progestational stage as compared with proestrous and estrous stage.<BR>Free amino acids in intact animal's serum were measured in proestrus, 5th day and 14th day of pregnancy (Fig. 2).<BR>Argine, threonine and glycine in serum decreased markedly according to stages of pregnancy. It seemed that there was no relation between the increase of free amino acid of uterine fluid and the decrease of free animo acid in serum during pregnancy.
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