One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
スポンサーリンク
概要
- 論文の詳細を見る
Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes simultaneously, and these mutations were normally inherited in the next generation.
- 日本繁殖生物学会の論文
著者
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SUGIURA Koji
Department of Applied Genetics, Graduate School of Agricultural and Life Sciences, University of Tok
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Fujii Wataru
Department Of Animal Science Graduate School Of Natural Science And Technology Okayama University
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Naito Kunihiko
Department Of Animal Resource Sciences Graduate School Of Agricultural And Life Sciences The Univers
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NAITO Kunihiko
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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ONUMA Asuka
Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System