Effects of hypercalcemic state on isolated pancreatic acinar cells. An experimental study on the mechanism involved in pancreatitis caused by hyperparathyroidism.:—AN EXPERIMENTAL STUDY ON THE MECHANISM INVOLVED IN PANCREATITIS CAUSED BY HYPERPARATHYROIDI

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An experimental study on the effects of hypercalcemic state on the pancreas was pe-rformed. Isolated pancreatic acinar cells were prepared by the Amsterdam & Jamieson's method. The cells were incubated for 1 week at 37°C, under 5% CO2 and 95% O2.(1) The cells in hypercalcemic medium (11.4 mM) produced by addition of CaCl2 were compared to those in normal control medium (1.8 mM). An optical microscopic examination showed presence of damaged cells and diminished number of the cells after 1 week incubation in the hypercalcemic state. Cellular content of DNA and protein were decreased, while cellular content of amylase, release of amylase (per DNA content) and LDH from the cells were increased in the hypercalcemic state. (2) Cellular content of DNA, protein and amylase decreased by administration of the CCK (3.3×10-7M) and did not decrease by additional administration of the CR1505 (2.5×10-6 W/V %), a CCK receptor antagonist, while release of amylase and LDH from the cells increased by administration of the CCK and did not increase by addition of CR1505 at the same time. These phenomena were more signifi-cant in the hypercalcemic state than in the normocalcemic state.The above mentioned data suggest that hypercalcemic state has a possibility of causing pancreatitis.
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