Chromosomal analysis in chorionic villi cultures from spontaneous abortions.
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Cytogenetic studies on the chorionic villus from spontaneous abortions of 5-1 week pregnancies were carried out to investigate the correlation between chromosome anomalies and spontaneous abortion. The method to be used as prenatal diagnosis was discussed. Chromosomes were prepared in long term culture and then were analysed by Q and G banding method. The frequency of chromosome anomalies detected totaled 55.7% (34 of 61 cases) investigated. The types of anomaly were classified as autosomal trisomies in 19 of these abnormal cases (55.9%), as polyploidy in 6 cases (17.6%), as structural anomalies in 5 (14.7%) and as monosomy X in the remaining 4 (11.8%). Diagnostic errors arising from examination of long term cultures of villus were found mostly due to maternal cell contamination. To overcome this problem, Y-body analysis, in situ harvesting and histological study were carried out. Y-body analysis and in situ harvesting were found useful for minimizing diagnostic errors. However, histological criteria were found insufficient for chromosomally abnormal diagnosis.
- 一般社団法人 国立医療学会の論文
一般社団法人 国立医療学会 | 論文
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