Epidemiological Survey of Streptococcus mutans among Japanese Children:Identification and Serological Typing of the Isolated Strains
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概要
- 論文の詳細を見る
An epidemiological investigation was carried out to identify and determine the serotypes of Streptococcus mutans from carious lesions of young Japanese children. For this purpose, a direct fluo-rescent antibody technique was mainly used. Fluorescein isothiocyanate-conjugated antibodies were prepared for the five known serotypes of S. mutans. Cross reactions and nonspecific reactions were eliminated by adsorption, counterstaining, or DEAE-cellulose column chromatography. Agar-gel immunodiffusion was used to distinguish between serotypes a and d. The epidemiological survey suggested that serotype c strains were most prevalent in dental plaques of Japanese children. The d and e serotypes were rare and serotypes a and b were not detected. It was also noted that more than one serotype of S. mutans could be found in the same locus of a carious lesion and that there might be no relationship between the degree of caries and the causative serotype(s) of S. mutans.
- 財団法人 学会誌刊行センターの論文
著者
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HAMADA SHIGEYUKI
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University
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KOTANI Shozo
Department of Microbiology and Oral Microbiology, Osaka University Dental School
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Sobue Shizuo
Department Of Pediatric Dentistry Osaka University Graduate School Of Dentistry
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Ooshima Takashi
Department Of Pediatric Dentistry Division Of Oral Infection And Disease Control Osaka University Gr
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Masuda Norio
Department Of Physics Faculty Of Science Tokai University
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HAMADA Shigeyuki
Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
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