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Developmental ability and viability after deep freezing of the blastomeres of mouse and rabbit embryos isolated at 2-cell stage were investigated by culturing these blastomeres in vitro. The blastomeres used were obtained by microsurgically dividing the 2-cell stage embryos along a inter-blastomeric groove with a fine glass needle. The zonae pellucidae were remained open or absent during the culture and the storage period.<BR>The successful dichotomies, which gave a pair of surviving blastomeres, were achieved in 81 out of 114 (71.7%) 2-cell embryos of mice and in 43 out of 62 (69.4%) 2-cell embryos of rabbits.;Forty-four out of 48 (91.7%) rabbit blastomeres developed into morphologically normal blasto-cysts, though they were significantly smaller in size than the respective control blastocysts derived from intact 2-cell embryos. In the mouse, 33 out of 87 (37.9%) blastomeres developed into 2-cell stage in culture.<BR>The mouse blastomeres survived after deep freezing at -80C. Ninety-one out of 100 (91.0%) blastomeres maintained normal morphology after freezing and thawing. Of these 91 blastomeres recovered in normal appearance, 73 (80.2%) developed in vitro into the 2-cell stage. In the rabbit, 28 out of 80 (35.0%) blastomeres were recovered in normal shape but only in one case a cleavage division into the 2-cell stage was observed after thawing.
日本繁殖生物学会の論文